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ACQUISITION WORKSPACE

Widefield fluorescence

PREVIEW

Recorded cells · 60-plane volumeLoading recorded specimen…

Explore the instrument

Excitation in. Fluorescence out.

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Shutter closed · illustrated pathDashed guides show where light would travel

Preparing the optical assembly…

01 · Excitation source

Inspect a component

Instrument source power8 %

Provides widefield excitation. The illuminated field is not raster-scanned.

Illustrative cells; not a one-to-one match to the microscope image.

Blue: excitationGreen: returning fluorescenceDashed paths: illustration only

Expanded teaching layout · nearby hardware omitted in close-ups · relay optics condensed · directions and scanning exaggerated. Mounts and glass are illustrative, not a manufacturer’s instrument.

Show optical path

Drag to rotate · choose a component
Hardware controls and the image use the same settings

Display settings

AutoscaleGamma1.00

Display changes do not alter raw detector values.

Setup check

Recorded source: 260 nm XY sampling, 290 nm Z spacing. Optical estimates do not guarantee this source resolves that detail.