LFMlab

LIGHT & FLUORESCENCE MICROSCOPY

JP Innovations · Training edition

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Instrument controls

Optics

Imaging modeObjectiveImmersion mediumFocus · Z position1.50 µmScan zoom1.00 ×

Focus through the specimen. Increasing magnification alone does not improve optical resolution.

Shutter closed · sample protected

ACQUISITION WORKSPACE

Widefield fluorescence

PREVIEW

384² preview

Recorded cells · 60-plane volumeLoading recorded specimen…

Explore the instrument

Light-path X-ray

Drag to orbit · click a component
Schematic excitation and emission paths

Display settings

AutoscaleGamma1.00

Display changes do not alter raw detector values.

Setup check

Recorded source: 260 nm XY sampling, 290 nm Z spacing. Optical estimates do not guarantee this source resolves that detail.

Ideal optical estimate ≈374 nm

Optical section ≈1.64 µm

Pixel / Nyquist target127 / 163 nm

Accumulated dose0.0000 J/cm²

Fluorescence remaining100.0 %

Nominal frame time160 ms